evaluation of economical and rapid method of plant dna

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Evaluation of economical and rapid method of plant DNA ...

01-06-2017  This DNA extraction method is evaluated for brinjal (Solanummelongena L.), chilli (Capsicum annuum L.), rice (Oryza sativa L.) and tomato (Solanumlycopersicum L.) crop. Many other crop plants could also be amplified using the same DNA extraction method for

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Evaluation of economical and rapid method of plant DNA ...

Evaluation of economical and rapid method of plant DNA extraction for PCR analysis of different crops Sweta Sinha1 and Amarendra Kumar2* 1Department of Molecular Biology and Genetic Engineering, Bihar Agricultural University, Sabour, Bhagalpur-813210 (Bihar ), INDIA

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Evaluation of economical and rapid method of plant DNA ...

In the recent genomic era, polymerase chain reaction (PCR) has become a basic tool in molecular studies and the success of PCR depends upon the template DNA. PCR technique is quite robust and often unnecessary to extract high quality of DNA and hence crude DNA can be used as template for amplification. Therefore, we have evaluated NaOH-Tris DNA extraction method for PCR analysis

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Evaluation of economical and rapid method of plant DNA ...

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Protocol: a rapid and economical procedure ... - Plant Methods

14-01-2010  Research in plant molecular biology involves DNA purification on a daily basis. Although different commercial kits enable convenient extraction of high-quality DNA from E. coli cells, PCR and agarose gel samples as well as plant tissues, each kit is designed for a particular type of DNA extraction work, and the cost of purchasing these kits over a long run can be considerable.

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A Rapid and Economical Method for Efficient DNA Extraction ...

13-07-2015  A rapid, cost effective method of metagenomic DNA extraction from soil is a useful tool for environmental microbiology. The present work describes an improved method of DNA extraction namely “powdered glass method” from diverse soils. The method involves the use of sterile glass powder for cell lysis followed by addition of 1% powdered activated charcoal (PAC) as purifying agent to remove ...

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Protocol: a rapid and economical procedure for ...

Protocol: a rapid and economical procedure for purification of plasmid or plant DNA with diverse applications in plant biology Jian-Feng Li1,2*,LiLi1,2, Jen Sheen1,2 Abstract Research in plant molecular biology involves DNA purification on a daily basis. Although different commercial kits

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Economical and Rapid Method for Extracting Cotton Genomic DNA

ZHANG STEWART: ECONOMICAL, RAPID GENOMIC DNA EXTRACTION 194 based on PCR soon found vast application in plant genetics and breeding (Lee, 1995). To accommodate the need for PCR-based markers, a rapid, simple, and reliable DNA preparation method is required to provide high quality and quantity DNA for the analyses. Although numerous DNA extraction

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A modified mini-prep method for economical and rapid ...

03-09-2012  Molecular markers for map-based cloning, marker-assisted selection in crop breeding, and genetic studies require DNA isolation from a large number of plants in a short span of time. Here we describe a modified DNA extraction method that is economical in terms of cost, time and labour. The method allows DNA extraction from as little as 0.2–0.3 g of leaves that are homogenized in zipper ...

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Back to basics: an evaluation of NaOH and alternative ...

detection from plant samples, and genotyping for population genetics and epidemiology, rapid and dependable DNA extraction methods scalable from one to hundreds of samples are desirable. We evaluated several rapid extrac-tion methods (NaOH, Rapid one-step extraction (ROSE), Chelex 100, proteinase K) for their ability to obtain DNA of quantity ...

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A Rapid and Economical Method for Efficient DNA Extraction ...

13-07-2015  A rapid, cost effective method of metagenomic DNA extraction from soil is a useful tool for environmental microbiology. The present work describes an improved method of DNA extraction namely “powdered glass method” from diverse soils. The method involves the use of sterile glass powder for cell lysis followed by addition of 1% powdered activated charcoal (PAC) as purifying agent

Get price

A modified mini-prep method for economical and rapid ...

03-09-2012  Molecular markers for map-based cloning, marker-assisted selection in crop breeding, and genetic studies require DNA isolation from a large number of plants in a short span of time. Here we describe a modified DNA extraction method that is economical in terms of cost, time and labour. The method allows DNA extraction from as little as 0.2–0.3 g of leaves that are homogenized in zipper ...

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(PDF) A modified mini-prep method for economical and rapid ...

Materials, methods, and results. Key words: DNA extraction method, cotton, molecular markers, wheat *Author for correspondence, e-mail: [email protected]; ph: +92-41-624892. tEditor's note: Although the scientific content of this article has been reviewed, the full-text Web publication has not been edited in detail.Table 1 .1Comparison of standard CTAB method with m o d i f i e d DNA ...

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Development of a rapid and efficient method for DNA ...

Several methods for extraction of genomic DNA from plant tissues have been carried out and demonstrated so far. Also this subject has been developed by improvement of science [2,3,4]. We have now modified the CTAB method for rapid isolation of DNA from plant. The DNA is suitable for ISSR analysis and other PCR-based applications. Materials and ...

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Evaluation of rapid and simple techniques for the ...

01-01-2014  Evaluation of rapid and simple techniques for the enrichment of viruses prior to metagenomic virus discovery. ... Development of a method for rapid sequencing of whole genomes from new viruses: ... Exploring the diversity of plant DNA viruses and their satellites using vector-enabled metagenomics on whiteflies. PLoS One, 6 ...

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A Rapid, Highly Efficient and Economical Method of ...

08-01-2014  Transient transformation is simpler, more efficient and economical in analyzing protein subcellular localization than stable transformation. Fluorescent fusion proteins were often used in transient transformation to follow the in vivo behavior of proteins. Onion epidermis, which has large, living and transparent cells in a monolayer, is suitable to visualize fluorescent fusion proteins.

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A Rapid Efficient Method for DNA Isolation from Plants ...

A Rapid Efficient Method for DNA Isolation from Plants with High Levels of Secondary Metabolites Asian Journal of Plant Sciences: Volume 6 (6): 977-981, 2007. A. Dehestani and S.K. Kazemi Tabar. Abstract. This research was designated to optimize a rapid, inexpensive method to isolate desirable DNA from plants with high concentrations of ...

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An evaluation of new and established methods to determine ...

A rapid and reliable evaluation method of the transgenic allele for copy number and homozygosity is vital in analysing these transformations. Here the suitability of Southern blot analysis, thermal asymmetric interlaced (TAIL-)PCR, quantitative (q)PCR and digital droplet (dd)PCR to estimate T-DNA copy number, locus complexity and homozygosity were compared in transgenic tobacco.

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rapid method of preparing megabase plant DNA Nucleic ...

François Guidet, Peter Rogowsky, Peter Langridge; A rapid method of preparing megabase plant DNA, Nucleic Acids Research, Volume 18, Issue 16, 25 August 1990, P

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(PDF) Evaluation of a modified salt-out method for DNA ...

Evaluation of a modified salt-out method for DNA extraction from whole blood lymphocytes: A simple and economical method for gene polymorphism December

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A Rapid and Economical Method for Efficient DNA Extraction ...

Besides providing rapid, efficient and economical extraction of metgenomic DNA from diverse soils, this method’s applicability is also demonstrated for cultivated organisms (Gram positive B. subtilis NRRL-B-201, Gram negative E. coli MTCC40, and a microalgae C. sorokiniana UTEX#1666).

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Development of a rapid and efficient method for DNA ...

Several methods for extraction of genomic DNA from plant tissues have been carried out and demonstrated so far. Also this subject has been developed by improvement of science [2,3,4]. We have now modified the CTAB method for rapid isolation of DNA from plant. The DNA is suitable for ISSR analysis and other PCR-based applications. Materials and ...

Get price

A Rapid, Highly Efficient and Economical Method of ...

08-01-2014  Transient transformation is simpler, more efficient and economical in analyzing protein subcellular localization than stable transformation. Fluorescent fusion proteins were often used in transient transformation to follow the in vivo behavior of proteins. Onion epidermis, which has large, living and transparent cells in a monolayer, is suitable to visualize fluorescent fusion proteins.

Get price

Evaluation of a rapid DNA extraction method to detect ...

Evaluation of a rapid DNA extraction method to detect yeast cells by PCR in orange juice Maria Ros-Chumillas a, Marcos Egea-Cortines b, Antonio Lopez-Gomez a, Julia Weiss b,* a Food and ...

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A Rapid Efficient Method for DNA Isolation from Plants ...

A Rapid Efficient Method for DNA Isolation from Plants with High Levels of Secondary Metabolites Asian Journal of Plant Sciences: Volume 6 (6): 977-981, 2007. A. Dehestani and S.K. Kazemi Tabar. Abstract. This research was designated to optimize a rapid, inexpensive method to isolate desirable DNA from plants with high concentrations of ...

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An alternative, rapid method of plant DNA extraction for ...

Semantic Scholar extracted view of "An alternative, rapid method of plant DNA extraction for PCR analyses." by D. Brunel

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rapid method of preparing megabase plant DNA Nucleic ...

François Guidet, Peter Rogowsky, Peter Langridge; A rapid method of preparing megabase plant DNA, Nucleic Acids Research, Volume 18, Issue 16, 25 August 1990, P

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(PDF) Evaluation of a modified salt-out method for DNA ...

Evaluation of a modified salt-out method for DNA extraction from whole blood lymphocytes: A simple and economical method for gene polymorphism December

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Available online at pbr.mazums.ac.ir PBR

Evaluation of a modified salt-out method for DNA extraction from whole blood lymphocytes: A simple and economical method for gene polymorphism Mohammad Shokrzadeh1, Abbas Mohammadpour1* 1Pharmaceutical Research Center, Department of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran

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CN103966198B - A kind of rapid extracting method of DNA of ...

The invention discloses the extracting method of a kind of DNA of plants.This Method of Plant DNA Extraction (ice bath method), comprises the steps 1) and 2): 1) crush plant organ and/or tissue, obtain plant powder;2) in described plant powder, add cell pyrolysis liquid and solution S is reacted 15 40 minutes in ice bath, obtain the extract containing DNA, this extract is referred to as ...

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